Temperature-dependence of Hematoporphyrin Derivative (HpD) -Uptake and Cytotoxicity with Hyperthermia

Laser-induced Fluorescence Studies of Hematoporphyrin Derivative (hpd) In Normal and Tumor-tissue of Rat

Temperature dependence studies of adriamycin uptake and cytotoxicity.

In order to learn whether a direct relationship exists between cellular uptake and cytotoxicity of Adriamycin, we have compared the temperature dependencies of these two processes in L1210 cells. We find that the equilibrium concentration of drug taken inside the cells varies smoothly with temperature between 37 degrees C and 0 degree C. Even at 0 degree C, however, there is still measurable up...

Pharmacological modulation of photodynamic therapy with hematoporphyrin derivative and light.

The interactions between photodynamic therapy (PDT) with hemotoporphyrin derivative (HPD) and treatment with cytotoxic drugs have been examined using both an in vitro tissue culture assay and an in vivo transplantable mouse tumor assay. Adriamycin (0.5-4.0 mg/kg) administered with HPD and at the time of irradiation potentiated the photodynamic effect, doubling the duration of tumor control. Adr...

Oxygen dependence of the photosensitizing effect of hematoporphyrin derivative in NHIK 3025 cells.

Cells of the established line NHIK 3025 were incubated with hematoporphyrin derivative and exposed to light at different concentrations of oxygen. The efficiency of photoinactivation of the sensitized cells decreased with decreasing oxygen concentration. No photoinactivation was observed when the atmosphere above the medium overlying the cells was pure N2 gas. With 1% O2 in the atmosphere, the ...

Uptake of hematoporphyrin derivative by valvular vegetations in experimental infective endocarditis.

Drugs that localize in valvular vegetations may be useful in the diagnosis and treatment of infective endocarditis. We therefore tested the hypothesis that parenterally injected hematoporphyrin derivative (HPD), which is concentrated in tumors and atherosclerotic plaques, localizes in the vegetations of experimental infective endocarditis. In 14 rabbits, various bacteria were given intra-arteri...